[ACEsthetics] Fw: Detection of oral bacterial DNA in synovial fluid -

http://onlinelibrary.wiley.com/doi/10.1111/jcpe.12102/abstract

Which is why ozone injections into joints or near joints may help the inflammatory process resolve.

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Question, though, does some other source of inflammation MAKE the joint more susceptible to bacterial entry and then they just show up along for the ride? Or, are they the source of the inflammation in the first place?

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Do inflammatory processes going on in the mouth MAKE the inflammation increase in the rest of the body even without the bacterial involvement distant to the mouth?

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Don't know if I've already mentioned that Ziad Fahmy, from Germany, has a new text out on uses of ozone in orthopedics and rheumatology. Very nicely illustrated. I got a couple extra copies when he spoke out at the IAOHD meeting if anyone's interested. Contact OFF list.

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Also think Eric Zaremski has a few.

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regards

bill domb

Detection of oral bacterial DNA in synovial fluid

1. Stefan Reichert^1,*,
2. Maximilian Haffner¹,
3. Gernot Keyßer²,
4. Christoph Schäfer²,
5. Jamal Stein³,
6. Hans-Guenter Schaller¹,
7. Andreas Wienke⁴,
8. Heiko Strauss⁵,
9. Steffen Heide⁵,
10. Susanne Schulz¹

Article first published online: 28 MAR 2013

DOI: 10.1111/jcpe.12102

© 2013 John Wiley & Sons A/S

Objectives

As periodontal bacteria might be involved in the aetiology of rheumatic diseases, we analysed synovial fluid obtained from patients with rheumatoid arthritis (RA) and controls for the presence of DNA of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Treponema denticola.

Methods

In all, 42 patients suffering from RA (mean age 53.8 ± 16.7 years, 40.4% females) and 114 controls with no rheumatic diseases (mean age 56.1 ± 15.2 years, 52.4% females) were included. DNA from synovial fluid was isolated by QiaAmp kit (Qiagen, Hilden, Germany). Polymerase chain reactions (PCRs) specific for the 16S rRNA genes of the above specified bacteria were developed. Subgingival bacterial colonization was analysed using micro-Ident^® test (HAIN-Diagnostik, Nehren, Germany).

Results

In patients with RA DNA of P. gingivalis was detected in synovial fluid more often than in controls (15.7% versus 3.5%, p = 0.045). More patients than controls harboured DNA from P. gingivalis in both, oral plaque and synovial fluid (11.9% versus. 0.9%, p = 0.030). Among the patients group the number of missing teeth was correlated with the number of joints with movement restrictions caused by RA.

Conclusions

DNA of periodontopathogens can be found in synovial fluid and oral bacteria may play a role in the pathogenesis of arthritis.